RECAPITI

• Via Prov.le Lecce-Monteroni, I73100 LECCE

ATTREZZATURE

• La principale attrezzatura del Laboratorio è costituita da: spettrofotometri, centrifughe da banco e da pavimento, ultracentrifuga, scintillatore, analizzatore di immagini, cappa sterile, cappa a flusso laminare, incubatori, termociclatori, apparati per elettroforesi e western blotting, HPLC, ossigrafo.

LINEE DI RICERCA

ATTIVITÀ / FINALITÀ

• Attività di ricerca e di formazione

TERZA MISSIONE

PERSONE

• Zara Vincenzo (Professore Ordinario)
• Capobianco Loredana (Professore Associato)
• Ferramosca Alessandra (Ricercatore Confermato)
• Giudetti Anna Maria (Ricercatore Confermato)
• Moscatelli Natalina (Dottorando)
• Pezzuto Francesca (Dottorando)

COLLABORAZIONI ESTERNE

• Jason Young, , McGill University, Montreal, Canada
• Joachim Rassow, Ruhr-Universität Bochum, Bochum, Germany
• Dennis Winge, Utah University, Salt Lake City, UT, USA
• Peter Rehling, Universität Göttingen, Göttingen, Germany
• Roland Lill, Philipps-Universität Marburg, Marburg, Germany
• Paul Schanda, Institut de Biologie Structurale Grenoble,France.

FORMAZIONE

• Corso di Laurea Triennale in Biologia: BIOCHIMICA (9 CFU);
• Corso di Laurea Magistrale in Biologia: BIOCHIMICA II (9 CFU);
• Corso di Laurea Magistrale in Biologia: BIOCHIMICA APPLICATA E DIAGNOSTICA (6 CFU)
• Corso di Laurea Magistrale in Biologia: SCIENZA DELL’ALIMENTAZIONE: PRINCIPI ED APPLICAZIONI (6 CFU)
• Corso di Laurea Triennale in Biotecnologie: BIOCHIMICA ed ENZIMOLOGIA (8 CFU)
• Corso di Laurea Magistrale in Biotecnologie Mediche e Nanobiotecnologie:  BIOTECNOLOGIE BIOCHIMICHE E BIOMOLECOLARI (Modulo di Biotecnologie Biochimiche 6 CFU)
   

PROGETTI

• Obesità e infertilità maschile: ruolo degli acidi grassi nella modulazione del metabolismo energetico degli spermatozoi”, finanziato dalla Fondazione Cassa di Risparmio di Puglia
   

PUBBLICAZIONI

• D Iacopetta, R Lappano, AR Cappello, M Madeo, E De Francesco, A Santoro, R Curcio, L Capobianco, V Pezzi, M Maggiolini, V Dolce. “SLC37A1 gene expression is up-regulated by epidermal growth factor in breast cancer cells.” (2010) Breast Cancer Res Treat, 122, 755-764.
• D Iacopetta, C Carrisi, G De Filippis, VM Calcagnile, AR Cappello, A Chimento, R Curcio, A Santoro, A Vozza, V Dolce, F Palmieri, L Capobianco. “The biochemical properties of the mitochondrial thiamine pyrophosphate carrier from Drosophila melanogaster.” (2010) FEBS J, 277, 1172-1181.
• GV Gnoni, AM Giudetti, E Mercuri, F Damiano, E Stanca, P Priore, L Siculella. “Reduced activity and expression of mitochondrial citrate carrier in streptozotocin-induced diabetic rats.” (2010) Endocrinology, 151, 1551-1559.
• G Serviddio, F Bellanti, AM Giudetti, GV Gnoni, A Petrella, R Tamborra, AD Romano, T Rollo, G Vendemiale, E Altomare. “A silybin-phospholipid complex prevents mitochondrial dysfunction in a rodent model of nonalcoholic steatohepatitis.” (2010) J Pharmacol Exp Ther, 332, 922-932.
• A Stendardi, R Focarelli, P Piomboni, D Palumberi, F Serafini, A Ferramosca, V Zara. Evaluation of mitochondrial respiratory efficiency during in vitro capacitation of human spermatozoa. (2011) Int J Androl, 34, 247-255.
• V Zara, L Conte. The Rieske Iron-Sulfur protein: Import and assembly into the cytochrome bc(1) complex of yeast mitochondria. (2011) Bioinorg Chem Appl, 2011:363941.
• A Ferramosca, L Conte, V Zara. A krill oil supplemented diet reduces the activities of the mitochondrial tricarboxylate carrier and of the cytosolic lipogenic enzymes in rats. (2012) J Anim Physiol Anim Nutr, 96, 295-306.
• L Conte, BL Trumpower, V Zara. Bcs1p can rescue a large and productive cytochrome bc(1) complex assembly intermediate in the inner membrane of yeast mitochondria. (2011) Biochim Biophys Acta, 1813, 91-101.
• D Iacopetta, M Madeo, G Tasco, C Carrisi, R Curcio, E Martello, R Casadio, L Capobianco, V Dolce. “A novel subfamily of mitochondrial dicarboxylate carriers from Drosophila melanogaster: biochemical and computational studies.” (2011) BBA-Bioenergetics, 1807, 251-261.
• L Capobianco, D Iacopetta, C Carrisi, M Madeo, AR Cappello, V Dolce. “An effective strategy for cloning the mitochondrial citrate carrier: identification, characterization and tissue distribution in silver eel.” (2011) Advances in Bioscience and Biotechnology, 2, 157-162.
• G Serviddio, F Bellanti, AM Giudetti, GV Gnoni, N Capitanio, R Tamborra, AD Romano, M Quinto, M Blonda, G Vendemiale, E Altomare. “Mitochondrial oxidative stress and respiratory chain dysfunction account for liver toxicity during amiodarone but not dronedarone administration.” (2011) Free Radic Biol Med, 51, 2234-2242.
• G Serviddio, AM Giudetti, F Bellanti, P Priore, T Rollo, R Tamborra, L Siculella, G Vendemiale, E Altomare, GV Gnoni. “Oxidation of hepatic carnitine palmitoyl transferase-I (CPT-I) impairs fatty acid beta-oxidation in rats fed a methionine-choline deficient diet.” (2011) PLoS ONE, 6(9): e24084.
• P Piomboni, R Focarelli, A Stendardi, A Ferramosca, V Zara. The role of mitochondria in energy production for human sperm motility. (2012) Int J Androl, 35, 109-124.
• A Ferramosca, S Pinto Provenzano, L Coppola, V Zara. Mitochondrial respiratory efficiency is positively correlated with human sperm motility. (2012) Urology, 79, 809-814.
• A Ferramosca, A Conte, L Burri, K Berge, F De Nuccio, AM Giudetti, V Zara. A krill oil supplemented diet suppresses hepatic steatosis in high-fat fed rats. (2012) PLoS ONE, 7 (6): e38797.
• AR Cappello, C Guido, A Santoro, M Santoro, L Capobianco, D Montanaro, A Madeo, S Andò, V Dolce, S Aquila. “The mitochondrial citrate carrier (CIC) is present and regulates insulin secretion by human male gamete.” (2012) Endocrinology, 153, 1743-1754.
• AM Giudetti, R Cagnazzo. “Beneficial effects of n-3 PUFA on chronic airway inflammatory diseases.” (2012) Prostaglandins Other Lipid Mediat, 99:57-67.
• A Ferramosca, V Zara. Biogenesis of mitochondrial carrier proteins: Molecular mechanisms of import into mitochondria. (2013) Biochim Biophys Acta, 1833, 494-502.
• A Ferramosca, S Pinto Provenzano, L Coppola, DD Montagna, V Zara. Oxidative stress negatively affects human sperm mitochondrial respiration. (2013) Urology, 82, 78-83.
• E De Franchis, A Ferramosca, V Zara, L Piper. Incidence of risk factors on the onset of gestational diabetes mellitus: an empirical research in southern Italy. (2013) International Journal of Measurement Technologies and Instrumentation Engineering, 2, 8-22.
• P Lunetti, AR Cappello, RM Marsano, CL Pierri, C Carrisi, E Martello, C Caggese, V Dolce, L Capobianco. “Mitochondrial glutamate carriers from Drosophila melanogaster: biochemical, evolutionary and modeling studies.” (2013) BBA-Bioenergetics, 1827, 1245-1255.
• E Stanca, G Serviddio, F Bellanti, G Vendemiale, L Siculella, AM Giudetti. “Down-regulation of LPCAT expression increases platelet-activating factor level in cirrhotic rat liver: potential antiinflammatory effect of silybin.” (2013) Biochim Biophys Acta, 1832, 2019-2026.
• AM Giudetti, F Damiano, GV Gnoni, L Siculella. “Low level of hydrogen peroxide induces lipid synthesis in BRL-3A cells through a CAP-independent SREBP-1a activation.” (2013) Int J Biochem Cell Biol, 45, 1419-1426.
• F Bellanti, AD Romano, AM Giudetti, T Rollo, M Blonda, R Tamborra, G Vendemiale, G Serviddio. “Many faces of mitochondrial uncoupling during age: damage or defense?” (2013) J Gerontol A Biol Sci Med Sci, 68, 892-902.
• T Cui, A Conte, JL Fox, V Zara, DR Winge. “Modulation of the respiratory supercomplexes in yeast: Enhanced formation of cytochrome oxidase increases the stability and abundance of respiratory supercomplexes”. (2014) J Biol Chem, 289, 6133-6141.
• A Ferramosca, V Zara. Modulation of hepatic steatosis by dietary fatty acids. (2014) World Journal of Gastroenterology, 20, 1746-1755.
• A Ferramosca, V Zara. Bioenergetics of mammalian sperm capacitation. (2014) Biomed Research International, 2014:902953. 
• A Ferramosca, V Zara. Dietary fat and hepatic lipogenesis: the mitochondrial citrate carrier as a sensor of metabolic changes. (2014) Advances in Nutrition, 5, 217-225.
• A Ferramosca, A Conte, F Damiano, L Siculella, V Zara. Differential effects of high carbohydrate and high fat diets on hepatic lipogenesis in rats. (2014) European Journal of Nutrition, 53, 1103-1114.
• S Felline, E Mollo, A Ferramosca, V Zara, F Regoli, S Gorbi, A Terlizzi. Can a marine pest reduce the nutritional value of Mediterranean fish flesh? (2014) Marine Biology, 161, 1275-1283.
• C Carrisi, D Antonucci, P Lunetti, D Migoni, CR Girelli, V Dolce, FP Fanizzi, M Benedetti, L Capobianco. “Transport of Platinum bonded nucleotides into proteoliposomes, mediated by Drosophila melanogaster thiamine pyrophosphate carrier protein (DmTpc1).” (2014) J Bioinorg Biochem, 130, 28-31.
• G Serviddio, F Bellanti, E Stanca, P Lunetti, M Blonda, R Tamborra, L Siculella, G Vendemiale, L Capobianco, AM Giudetti. “Silybin exerts antioxidant effects and induces mitochondrial biogenesis in liver of rat with secondary biliary cirrhosis.” (2014) Free Radic Biol Med, 73, 117-126.
• V Dolce, AR Cappello, L Capobianco. Mitochondrial Tricarboxylate and Dicarboxylate–Tricarboxylate Carriers: from animals to Plants. (2014) IUBMB life, 66, 462- 471.
• D Vergara, AM Giudetti, D Latorre, P Lunetti,  P Simeone, D Lofrumento, F Denuccio, G Nicolardi, L Capobianco, M Maffia. “Molecular and biochemical alterations in epithelial to mesenchymal transition.” (2014) J Biotechnol.
• A Ferramosca, A Conte, V Zara. “Krill oil ameliorates mitochondrial dysfunctions in rats treated with high-fat diet”. (2015) Biomed Res Int, 2015:645984.
• A Conte, B Papa, A Ferramosca, V Zara. “The dimerization of the yeast cytochrome bc1 complex is an early event and is independent of Rip1”. (2015) Biochim Biophys Acta, 1853, 987-995.
• L De Riccardis, A Rizzello, A Ferramosca, E Urso, F De Robertis, A Danieli, AM Giudetti, G Trianni, V Zara, M Maffia. “Bioenergetics profile of CD4+ T cells in relapsing remitting Multiple Sclerosis subjects.” (2015) J Biotechnol, 202: 31-39.
• A Ferramosca, D Albani, L Coppola, V Zara. “Varicocele negatively affects sperm mitochondrial respiration.” (2015) Urology, 86, 735-739.
• D Vergara, P Simeone, D Latorre, F Cascione, S Leporatti, M Trerotola, AM Giudetti, L Capobianco, P Lunetti, A Rizzello, R Rinaldi, S Alberti, M Maffia. “Proteomics analysis of E-Cadherin knockdown in epithelial breast cancer cells.” (2015) J Biotechnol, 202, 3-11.
• D Vergara, M D'Alessandro, A Rizzello, L De Riccardis, P Lunetti, P Del Boccio, F De Robertis, G Trianni, M Maffia, AM Giudetti. “A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis.” (2015) BMC Neurosci, 24, 16:46.
• A Aloisi, E Tarentini, A Ferramosca, V Zara, R Rinaldi. “Micro-oxygraph device for biosensoristic applications.” (2015) J Sensors, in press.
• D Vergara, P Simeone,  F Julien, M Trerotola,  A Giudetti, L Capobianco, A Tinelli, C Bellomo, I Fournier, A Gaballo, S Alberti, M Salzet, M Maffia. “Translating epithelial mesenchymal transition markers into the clinic: novel insights from proteomics.” (2015) EuPA Open Proteomics, in press.
• AM Giudetti, A Romano, AM Lavecchia, S Gaetani. “The role of brain cholesterol and its oxidized products in Alzheimer's disease.” (2015) Curr Alzheimer Res, in press.
   

BREVETTI

• AWC Einerhand, L Gambelli, U Schmid, V Zara. “Pinolenic acid compositions for treating fatty liver.” EP2002730 (A1)
• AWC Einerhand, L Gambelli, U Schmid, V Zara. “Use of pinolenic acid for increasing lean body mass.” EP2002731 (A1)
• AWC Einerhand, L Gambelli, U Schmid, V Zara. “Use of pinolenic acid for lowering cholesterol.” EP2002729 (A1)
• A Aloisi, E Tarentini, G Leopizzi, R Rinaldi, A Ferramosca, V Zara. “Oxygraph chamber and insertable micro-culture device and uses thereof.” EP2597148 (A1)

CONSULENZE

• Effetto di nutrienti/nutraceutici sul metabolismo lipidico
• Infertilità maschile
• Bioenergetica mitocondriale in condizioni fisiologiche e patologiche
• Composizione lipidica di membrane cellulari in varie condizioni metaboliche e dismetaboliche

DOVE SIAMO

• Via Prov.le Lecce-Monteroni, I 73100 LECCE